In Situ Hybridization Protocols (Methods in Molecular Biology, 326)
ISBN-13:
9781617375446
ISBN-10:
1617375446
Edition:
Softcover reprint of hardcover 3rd ed. 2006
Author:
Ian A. Darby, Tim D. Hewitson
Publication date:
2010
Publisher:
Humana
Format:
Paperback
282 pages
Category:
Biochemistry
,
Chemistry
,
Molecular Biology
,
Evolution
,
Biological Sciences
FREE US shipping
Book details
ISBN-13:
9781617375446
ISBN-10:
1617375446
Edition:
Softcover reprint of hardcover 3rd ed. 2006
Author:
Ian A. Darby, Tim D. Hewitson
Publication date:
2010
Publisher:
Humana
Format:
Paperback
282 pages
Category:
Biochemistry
,
Chemistry
,
Molecular Biology
,
Evolution
,
Biological Sciences
Summary
In Situ Hybridization Protocols (Methods in Molecular Biology, 326) (ISBN-13: 9781617375446 and ISBN-10: 1617375446), written by authors
Ian A. Darby, Tim D. Hewitson, was published by Humana in 2010.
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Description
The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.
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