DNA Microarrays: A Molecular Cloning Manual
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Designed to extend and complement the information in Molecular Cloning , this laboratory guide synthesizes the expertise and experience of about 30 innovators in DNA microarray technology, which is used to analyze genomes and characterize patterns of gene expression. The contributions emphasize the use of cDNA microarrays for expression analysis and focus upon two currently dominant technologies spotted DNA microarrays on glass slides, and in situ synthesized oligonucleotide arrays. Topics include generation of probes for spotted microarrays, printing spotted glass microarrays, expression analysis of RNA, membrane-based spotted cDNA arrays, tissue microdissection, using microarrays for genomic analysis, an introduction to microarray bioinformatics, and tissue microarrays. Edited by Botwell and Samrook, both of the Peter MacCallum Cancer Institute. Annotation c. Book News, Inc., Portland, OR (booknews.com)
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