Essential Molecular Biology: A Practical Approach, Volume II

The two volumes of "Essential Molecular Biology: A Practical Approach" provide clear theoretical and practical guidance to the fundamental techniques of recombinant DNA analysis. Although of particular value to research workers entering the field for the first time, the protocols described will also be of interest to experienced researchers. Volume 2 covers the procedures needed for identification of specific genes and the study of their structure and cellular expression. Topics covered include preparation of gene libraries, hybridisation methods, nucleic acid sequencing, DNA mapping, DNA amplification by the polymerase chain reaction, and RNA transcript analysis. Each chapter is written in an accessible style by an acknowledged expert in that field with emphasis on sound technical instruction, in combination with adequate theoretical background and useful troubleshooting guidance. This book thus brings powerful techniques, at the forefront of molecular biology research within reach of any researcher. Together, the two volumes provide a comprehensive molecular biology manual tailored specifically for the novice. This volume is also available as part of a two-volume set with Volume 1.